Catalog Number - 85318-10P-2

The 85318‑10P‑2 Cogent RP C18 ABX™ HPLC Column is engineered for high‑resolution separations under extreme mobile phase conditions, providing stable performance across a wide pH range (2–12).

Built with a bulky end‑capped C18 bonded phase on high‑purity silica, this column minimizes unwanted interactions and improves peak shape for acidic, basic, and neutral analytes, especially those with extreme pKa values.

✅ Ideal as a USP L1 alternate for methods involving strong acids or bases

100 mm Length – Increased Resolution for LC‑MS Methods

• Higher resolving power than 50 mm columns

• Improves separation of closely related compounds

• Increases peak capacity for complex mixtures

• Ideal for method refinement following initial screening

✅ Selected when greater resolution is required beyond fast scouting methods

2.1 mm ID – LC‑MS / Low‑Flow Optimization

• Optimized for low‑flow HPLC and LC‑MS systems

• Enhances MS sensitivity due to reduced solvent load

Typical Flow Rate Range

• ~0.2–0.3 mL/min

✅ Requires precise, stable low‑flow pump capability

3 µm Particle – Balanced Efficiency & Pressure

• Higher efficiency than 5 µm columns

• Produces:

  • Sharper peaks

  • Improved resolution

• Lower backpressure than 1.8 µm UHPLC columns

✅ Ideal for:

• LC‑MS systems without UHPLC pressure limits

• High‑performance HPLC workflows

Wide pH Stability (pH 2–12) – Extreme Method Capability

• Designed for acidic and basic mobile phases

• Suitable for:

  • Strong acids

  • Strong bases

• Maintains column integrity under aggressive conditions

✅ Enables separations not achievable with standard C18 columns

Why Choose RP C18 ABX™ Columns (3 µm)

Extreme pH Ruggedness

• Stable across full pH range (2–12)

• Ideal for challenging analytes and harsh conditions

Balanced LC‑MS Performance

• Maintains efficiency without extreme backpressure

• Provides flexibility across HPLC and LC‑MS systems

Improved Peak Shape for Acids & Bases

• End‑capping reduces secondary interactions

• Enhances chromatographic reproducibility

✅ Excellent for method development and LC‑MS optimization

LC‑MS Performance Advantages

• Narrow‑bore 2.1 mm format:

  • Reduces solvent consumption

  • Improves sensitivity in MS detection

• 100 mm length:

  • Provides higher resolution than short columns

  • Supports complex mixture separations

✅ A preferred configuration for LC‑MS workflows requiring resolution and sensitivity

System & Sample Considerations

• Designed for low‑flow HPLC and LC‑MS systems

• Requires precise pump delivery and low‑volume detection systems

Recommended sample preparation:

• 👉 Use 0.2 µm syringe filters for proper sample preparation before injection

✅ Helps:

• Prevent column clogging

• Maintain long‑term performance

Typical Applications

• LC‑MS and LC‑MS/MS analysis

• Acidic and basic compound separations

• Pharmaceutical method development

• Stability‑indicating methods

• Peptide and polar compound analysis

• Complex mixture resolution under extreme pH

Important Usage Notes

• Designed for 2.1 mm LC‑MS and low‑flow systems (~0.2–0.3 mL/min)

• Suitable for extreme pH mobile phases (pH 2–12)

• Ideal for high‑resolution LC‑MS workflows

• Verify system capability for low‑flow stability and pressure limits